Investigation of Some Moraxella lacunata virulence Factors and Detection of Toxoplasma gondii in Patients with Eye Infections in Hilla City

Zahra a Abd Al-Aziz Yousif,Mohammed Sabri Abdul Razzaq, Hadi Fadil Al-Yasari
Authors Emails are requested on demand or by logging in
Keywords : Moraxella lacunata, Lysozyme, CFA/I and CFA/III
Medical Journal of Babylon  13:3 , 2016 doi:1812-156X-13-3
Published :31 December 2016

Abstract

Wilson disease (WD) is an autosomal recessive disorder in ATP7B gene which encodes a copper-transporting ATPase. This protein involves in the transport of copper into the plasma protein ceruloplasmin and in excretion of copper from the liver. Large numbers of mutations in ATP7B gene were found to have a role in the pathogenesis of WD. The variation in the clinical and biochemical features of the disease renders the diagnosis difficult task. This study aimed to determine the mutations in exon14 and 21 of ATP7B gene in Iraqi patients with WD to be used for molecular diagnosis.A total of 35 patients with WD and other 10 apparently healthy individuals were recruited for this study. Blood sample was obtained from each subject from which DNA was extracted and exon 14 and 21 were amplified with amplification refractory mutation system (ARMS) and conventional polymerase chain reaction using specific primers. Direct sequencing was used to find out the mutations in these exons.Five novel mutations and one single nucleotide polymorphism (homozygous or heterozygous) were recorded, among which 3 silent (c.3133C>T, c.4194T>C and c.4302G>A), 1 missense (c.3181G>C) and 2 splice site mutations (IVS21+40delA and IVS21+23G>A). The heterozygous mutation c.3133C>T was the most prevalent one among WD patients (40%). For diagnostic purpose, these results strongly suggested the heterozygous mutation c.3133C>T could be exploited in combination with the other high prevalent mutations

Introduction

Moraxella lacunata bacteria is isolated from eyes of patients suspected of infection as major ocular bacterial agent. Ocular bacterial infection present with varying in clinical symptoms and signs, these include pus discharge, visual impairment and conjunctival hyperemia. The infection by this bacteria may be acquired from external environment or from systemic infection by blood, which acts as transport media , also because this bacteria presented as a normal flora on the eyelid and conjunctiva for this any limited protect environment facilitated external or internal infection [1]. Many predisposing factors facilitated infection with, Moraxella lacunata especially patients who are alcoholic, diabetic or debilitated person, as well as contact lenses use help to increase keratitis infections [2]. There is little information about this species of Moraxella when compared to other Moraxella spp. particularly Moraxella catarrhals. However little is known about its virulence factors which are studied mostly by molecular technique [3]. Furthermore, the ocular infection can occur by parasite agent such as Toxoplasma gondii. The Toxoplasmosis can be transmission congenitally or postnatally, also may occur ocular lesion during some years after the acute infection[4]. Toxoplasma gondii is infected about more than third of world s population, also its answerable for the majority of uveitis infection (intra ocular infection), about 50% rate of posterior uveitis infection in many countries occur by acquired Toxoplasma [5,6].

Materials and methods

N/A

Results

Isolation of Moraxella lacunata: According to the data obtained in this study, only 12 isolates of M.lacunataare isolated from eye infection as show in Table (1), and the results showed that all the patients are free from ocular toxoplasmosis by detect the level of IgG and Ig M as shown in Table (2). The isolation rate is (12%) higher than that obtain by Mahdi [11]and Masri [12]. However, the presence of this bacteria in eye infection is not recorded previously in Hilla city.

Discussions

Isolation of Moraxella lacunata: According to the data obtained in this study, only 12 isolates of M.lacunataare isolated from eye infection as show in Table (1), and the results showed that all the patients are free from ocular toxoplasmosis by detect the level of IgG and Ig M as shown in Table (2). The isolation rate is (12%) higher than that obtain by Mahdi [11]and Masri [12]. However, the presence of this bacteria in eye infection is not recorded previously in Hilla city.

References

1- Willcox, M.D.P (2013). Characterization of the normal microbiota of the ocular surface . Experimental Eye Research 99-105 .
2- Das, S., Constatinou, M., Daniell, M and Taylor, H.R (2006). Moraxellakeratitis predisposing factors and clinical review of 95 cases. Br J Ophthalmol. 90(10) :1236-1238.
3- Sosa ,V., Umpierrez, A., Acquistapace, S. and Zunino, P. (2015). Virulence genes in Moraxella spp. isolates from infectious bovine keratoconjunctivitis cases. J Infect Dev Ctries., 9 (9) :1028-1032.
4-Commodaro, A. G., Blefort, R.N., Rizzo, V.L., Muccioli, C., Silveira, C., Burnierjr, M. N. and Belfort Jr, R. (2009). Ocular toxoplasmosis–an update and review of the literature. Mem Inst Oswaldo Cruz ,Rio de Janeiro, 104(2):345-350
5- Vallochi, A.L., Muccioli, C., Martins, M.C., Silveira, C., Blefort, R. JR. and Rizzo,L.V.(2005).The genotype of Toxoplasma gondii strain causing ocular toxoplasmosis in humans in Brazil. Am J Ophthalmol 139 : 350-361.
6- Soheilian,M., Heidari, K., Yazdani, S.,Shahsavari,M., Ahmadieh, H. and Dehghan, M.(2004). Patterns of uveitis In tertiary eye care center in Iraq.OculImmunol Inflamm 12: 297-310.
7- McFadden, J.F. (2000). Biochemical tests for the identification of medical bacteria. 3rdED.The Williams and Wilkins-Baltimor. USA. Medical Research. vol.139(1):91–98.
8- Winn, W. C., Allen, S. D., Janda, W. M., Koneman, E. W., Procop, G. W., Schreckenberger, P.C. and Woods, G. L.(2006). Koneman,s color atlas and textbook of diagnostic microbiology. 6th ED. Lippincott Williams and Wilkins,USA. PP:234-241.
9- Cockerill, F., Wikler, M., Bush, K., Dudley, M., Eliopoules, G.,Hardy, D., Hecht, D., Hindler, J., Patel, J., Powell, M., Thomson, R., Turnidge, J., Weinstein, M., Zimmer, B., Ferraro, M. and Swenson, J.(2010). Performance standards for antimicrobial susceptibility testing. Twentienth information supplement. 30(1):30-40.
10- Sambrook, J. and Russell, D.W. (2001). Molecular cloning: Alaboratory manual, 3rd ED,Cold Spring Harbor Laboratory Press. Cold Spring Harbor. NY. USA.
11-Mahdi, A,G. (2009). Isolation and identification of aerobic bacteria causing infection eyes of newborn babies in Al. Diwaniya city and it is sensitivity to some antibiotics. Journal of Karbala University, vol .7 No. 2 Scientific.
12- Masri, S. A. R. M. (2003). Isolation and identification of aerobic bacteria associated with eye infection in human. M.Sc. Thesis. University of Khartoum. Collage of Science
13- Kau, A.L., Hunstad, D. A. and Hultgren, S.J. (2005). Interaction of uropathognic Escherichia coli with host uroepithlium. Current opinion in Microbiol, 8(1): 54-59.
14- Dart, D.A and Willcox, M.D. P. (2013). Complexity of the tear film: importance in homeostasis and dysfunction during disease. Exp. Eye. Res 117: 1-3.


The complete article is available as a PDF File that is freely accessible. The fully formatted HTML version can be viewed as HTML Page.

Medical Journal of Babylon

volume 13 : 3

Share |

Viewing Options

Abstract
Download Abstract File
( 89 KB )

Related literature

Cited By
Google Blog Search
Other Articles by authors

Related articles/pages

On Google
On Google Scholar
On UOBabylon Rep

User Interaction

178  Users accessed this article in 1 year past
Last Access was at
14/12/2017 14:22:29